Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Lysozyme-Antimicrobial Peptide Fusion Protein Promotes the Diabetic Wound Size Reduction in Streptozotocin (STZ)-Induced Diabetic Rats
doi: 10.12659/MSM.912596
Figure Lengend Snippet: Fusion protein accelerated angiogenesis in wound tissue. ( A ) Serum levels of VEGF; ( B ) serum levels of ICAM-1; ( C ) expression of VEGF; ( D ) relative levels of VEGF; ( E ) expression of FGF-2; ( F ) relative levels of FGF-2; ( G ) number of small vessels, the small vessels were counted via CD34-positive staining. Results were expressed as mean ± standard error of the mean of the values. The number of animals per group was 8 for determination of VEGF, ICAM-1 content in serum, and 6 for expression of VEGF, and FGF-2. NORM-C – normal control; DIAB-C – diabetes control; DIAB-T – diabetes treated with the fusion protein. ** P <0.01 versus NORM-C; ## P <0.01 versus DIAB-C.
Article Snippet: After incubation with bovine serum albumin for blockade of non-specific binding, the sections were incubated rabbit polyclonal antibodies PDGF, CD34 primary antibody (Santa Cruz Biotechnology, USA) (1: 00) overnight at 4°C.
Techniques: Expressing, Staining, Control